Detailed information of TA system TA128756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 91283..91868
• Replicon: plasmid pJ9-3
• Accession: NZ_CP041590
• Organism: Acinetobacter baumannii strain J9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: FK728_RS18635
• Protein ID: WP_000897307.1
• Coordinates: 91548..91868 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FK728_RS18630
• Protein ID: WP_000369781.1
• Coordinates: 91283..91555 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK728_RS18615	87193..88283	+	1091	WP_085940413.1	IS4-like element ISAba1 family transposase	-
FK728_RS18620	88375..89259	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
FK728_RS18625	89315..90790	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
FK728_RS18630	91283..91555	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
FK728_RS18635	91548..91868	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FK728_RS18640	92021..92551	+	531	WP_000172759.1	hypothetical protein	-
FK728_RS18645	92820..93107	+	288	WP_000438825.1	BrnT family toxin	-
FK728_RS18650	93094..93408	+	315	WP_004728120.1	BrnA antitoxin family protein	-
FK728_RS18655	93475..93771	-	297	WP_111916319.1	MerR family DNA-binding protein	-
FK728_RS18660	93797..94180	+	384	WP_001055585.1	IS66 family insertion sequence hypothetical protein	-
FK728_RS18665	94177..94512	+	336	WP_000618092.1	IS66 family insertion sequence element accessory protein TnpB	-
FK728_RS18670	94587..96170	+	1584	WP_111916287.1	IS66-like element ISAba25 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / floR / blaADC-25 / mph(E) / msr(E)	-	1..145071	145071
	-	inside	IScluster/Tn	floR / blaADC-25 / mph(E) / msr(E)	-	79391..98850	19459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T128756 WP_000897307.1 NZ_CP041590:c91868-91548 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T128756 NZ_CP041590:c91868-91548 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT128756 WP_000369781.1 NZ_CP041590:c91555-91283 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT128756 NZ_CP041590:c91555-91283 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3