Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 5695761..5696411 Replicon chromosome
Accession NZ_CP041515
Organism Klebsiella michiganensis strain KNU07

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag FNA74_RS28825 Protein ID WP_014227314.1
Coordinates 5696070..5696411 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag FNA74_RS28820 Protein ID WP_014227313.1
Coordinates 5695761..5696060 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA74_RS28800 5691646..5692365 - 720 WP_009653851.1 phosphonate utilization transcriptional regulator PhnR -
FNA74_RS28805 5692506..5693609 + 1104 WP_064375121.1 2-aminoethylphosphonate--pyruvate transaminase -
FNA74_RS28810 5693619..5694428 + 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
FNA74_RS28815 5694461..5695642 - 1182 WP_064375120.1 purine ribonucleoside efflux pump NepI -
FNA74_RS28820 5695761..5696060 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
FNA74_RS28825 5696070..5696411 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FNA74_RS28830 5696662..5697114 - 453 WP_009653860.1 DUF1198 domain-containing protein -
FNA74_RS28835 5697292..5698683 - 1392 WP_064375119.1 hexose-6-phosphate:phosphate antiporter -
FNA74_RS28840 5698829..5700148 - 1320 WP_014227316.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5680456..5696411 15955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T128750 WP_014227314.1 NZ_CP041515:c5696411-5696070 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T128750 NZ_CP041515:c5696411-5696070 [Klebsiella michiganensis]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGTTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT128750 WP_014227313.1 NZ_CP041515:c5696060-5695761 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT128750 NZ_CP041515:c5696060-5695761 [Klebsiella michiganensis]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGTGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAGCTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References