Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3959193..3959695 Replicon chromosome
Accession NZ_CP041513
Organism Shigella boydii strain KCCM 41690

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FNA65_RS21415 Protein ID WP_000767829.1
Coordinates 3959441..3959695 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNA65_RS21410 Protein ID WP_001259255.1
Coordinates 3959193..3959444 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA65_RS21385 3954371..3955438 - 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FNA65_RS21390 3955438..3956508 - 1071 WP_039080504.1 histidinol-phosphate transaminase -
FNA65_RS21395 3956505..3957809 - 1305 WP_039080505.1 histidinol dehydrogenase -
FNA65_RS21400 3957815..3958714 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FNA65_RS21405 3958860..3958910 - 51 WP_001364200.1 his operon leader peptide -
FNA65_RS21410 3959193..3959444 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNA65_RS21415 3959441..3959695 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNA65_RS21420 3959778..3960602 + 825 WP_000754747.1 SDR family oxidoreductase -
FNA65_RS21425 3960648..3961577 + 930 WP_039059232.1 LysR family transcriptional regulator -
FNA65_RS21430 3961792..3961854 + 63 WP_010723108.1 membrane protein YoeI -
FNA65_RS21435 3961844..3963202 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNA65_RS21440 3963412..3964341 + 930 WP_024258582.1 YeeE/YedE family protein -
FNA65_RS21445 3964355..3964582 + 228 WP_000985274.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128725 WP_000767829.1 NZ_CP041513:3959441-3959695 [Shigella boydii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128725 NZ_CP041513:3959441-3959695 [Shigella boydii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128725 WP_001259255.1 NZ_CP041513:3959193-3959444 [Shigella boydii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128725 NZ_CP041513:3959193-3959444 [Shigella boydii]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCTAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References