Detailed information of TA system TA128722

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3770732..3770957
• Replicon: chromosome
• Accession: NZ_CP041513
• Organism: Shigella boydii strain KCCM 41690

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FNA65_RS20340
• Protein ID: WP_000935258.1
• Coordinates: 3770732..3770944 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3770899..3770957 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA65_RS20305	3765899..3767437	-	1539	WP_134796840.1	IS66-like element ISEc22 family transposase	-
FNA65_RS20310	3767486..3767833	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
FNA65_RS20315	3767830..3768210	-	381	WP_001333468.1	IS66 family insertion sequence hypothetical protein	-
FNA65_RS20320	3768565..3769692	-	1128	WP_001063816.1	IS110-like element ISSso6 family transposase	-
FNA65_RS20325	3769823..3770284	-	462	Protein_3713	hypothetical protein	-
FNA65_RS20330	3770286..3770564	-	279	WP_000929754.1	hypothetical protein	-
FNA65_RS20340	3770732..3770944	-	213	WP_000935258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3770899..3770957	+	59	-	-	Antitoxin
FNA65_RS20355	3771539..3771892	-	354	WP_071821547.1	hypothetical protein	-
FNA65_RS20360	3772025..3772405	+	381	WP_001333468.1	IS66 family insertion sequence hypothetical protein	-
FNA65_RS20365	3772402..3772749	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
FNA65_RS20370	3772798..3774336	+	1539	WP_134802545.1	IS66-like element ISEc22 family transposase	-
FNA65_RS20375	3774333..3774692	+	360	WP_073692993.1	3'-5' exoribonuclease	-
FNA65_RS20380	3774751..3774954	+	204	WP_000096344.1	DUF4224 domain-containing protein	-
FNA65_RS20385	3774954..3775309	+	356	Protein_3722	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rhs/PAAR / ipaH9.8	3653912..3789604	135692
	-	inside	IScluster/Tn	-	-	3763535..3776510	12975

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7856.42 Da
• Isoelectric Point: 8.7469

>T128722 WP_000935258.1 NZ_CP041513:c3770944-3770732 [Shigella boydii]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T128722 NZ_CP041513:c3770944-3770732 [Shigella boydii]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT128722 NZ_CP041513:3770899-3770957 [Shigella boydii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin