Detailed information of TA system TA128703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4168645..4169372
• Replicon: chromosome
• Accession: NZ_CP041511
• Organism: Shigella sonnei strain KCCM 41282

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: FNA55_RS21295
• Protein ID: WP_000547563.1
• Coordinates: 4169061..4169372 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FNA55_RS21290
• Protein ID: WP_000126296.1
• Coordinates: 4168645..4169064 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA55_RS21270 (4163696)	4163696..4164223	-	528	WP_001078777.1	electron transport protein HydN	-
FNA55_RS21275 (4164371)	4164371..4165381	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
FNA55_RS21280 (4165641)	4165641..4167098	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FNA55_RS21285 (4167107)	4167107..4168531	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
FNA55_RS21290 (4168645)	4168645..4169064	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
FNA55_RS21295 (4169061)	4169061..4169372	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
FNA55_RS21300 (4169534)	4169534..4170307	-	774	WP_001026444.1	hypothetical protein	-
FNA55_RS21305 (4170332)	4170332..4170802	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FNA55_RS21310 (4170795)	4170795..4171205	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
FNA55_RS21315 (4171202)	4171202..4171969	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
FNA55_RS21320 (4171969)	4171969..4172511	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
FNA55_RS21325 (4172521)	4172521..4174217	-	1697	Protein_4093	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T128703 WP_000547563.1 NZ_CP041511:c4169372-4169061 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T128703 NZ_CP041511:c4169372-4169061 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT128703 WP_000126296.1 NZ_CP041511:c4169064-4168645 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT128703 NZ_CP041511:c4169064-4168645 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22