Detailed information of TA system TA128685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3666653..3667347
• Replicon: chromosome
• Accession: NZ_CP041452
• Organism: Escherichia coli strain YPE3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FNP78_RS18605
• Protein ID: WP_001263493.1
• Coordinates: 3666653..3667051 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FNP78_RS18610
• Protein ID: WP_000554757.1
• Coordinates: 3667054..3667347 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP78_RS18575	3662212..3662670	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FNP78_RS18580	3662931..3664388	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FNP78_RS18585	3664445..3664966	-	522	Protein_3468	peptide chain release factor H	-
FNP78_RS18590	3664965..3665168	-	204	Protein_3469	RNA ligase RtcB family protein	-
FNP78_RS18595	3665414..3665866	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FNP78_RS18600	3665940..3666637	+	698	Protein_3471	IS1 family transposase	-
FNP78_RS18605	3666653..3667051	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FNP78_RS18610	3667054..3667347	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FNP78_RS18615	3667399..3668454	-	1056	WP_001226164.1	DNA polymerase IV	-
FNP78_RS18620	3668525..3669310	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
FNP78_RS18625	3669255..3670994	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
FNP78_RS18630	3671218..3671715	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3666344..3666637	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T128685 WP_001263493.1 NZ_CP041452:c3667051-3666653 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T128685 NZ_CP041452:c3667051-3666653 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT128685 WP_000554757.1 NZ_CP041452:c3667347-3667054 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT128685 NZ_CP041452:c3667347-3667054 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6