Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3808742..3809436 Replicon chromosome
Accession NZ_CP041448
Organism Escherichia coli strain YPE10

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag FNP79_RS19390 Protein ID WP_001263493.1
Coordinates 3808742..3809140 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag FNP79_RS19395 Protein ID WP_000554757.1
Coordinates 3809143..3809436 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNP79_RS19360 3803742..3804986 - 1245 WP_000189541.1 esterase FrsA -
- 3804402..3804482 - 81 NuclAT_11 - -
- 3804402..3804482 - 81 NuclAT_11 - -
- 3804402..3804482 - 81 NuclAT_11 - -
- 3804402..3804482 - 81 NuclAT_11 - -
FNP79_RS19365 3805078..3805536 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
FNP79_RS19370 3805797..3807254 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
FNP79_RS19375 3807311..3807832 - 522 Protein_3634 peptide chain release factor H -
FNP79_RS19380 3807831..3808034 - 204 Protein_3635 RNA ligase RtcB family protein -
FNP79_RS19385 3808280..3808732 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
FNP79_RS19390 3808742..3809140 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FNP79_RS19395 3809143..3809436 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FNP79_RS19400 3809488..3810543 - 1056 WP_001226164.1 DNA polymerase IV -
FNP79_RS19405 3810614..3811399 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
FNP79_RS19410 3811344..3813083 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
FNP79_RS19415 3813307..3813804 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3807780..3827388 19608


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T128663 WP_001263493.1 NZ_CP041448:c3809140-3808742 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T128663 NZ_CP041448:c3809140-3808742 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT128663 WP_000554757.1 NZ_CP041448:c3809436-3809143 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT128663 NZ_CP041448:c3809436-3809143 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EXB8


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References