Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3745246..3745787 Replicon chromosome
Accession NZ_CP041442
Organism Escherichia coli strain YPE12

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag FNP80_RS20045 Protein ID WP_000615983.1
Coordinates 3745509..3745787 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag FNP80_RS20040 Protein ID WP_000729703.1
Coordinates 3745246..3745506 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNP80_RS20020 3740921..3741706 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
FNP80_RS20025 3741651..3743390 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
FNP80_RS20030 3743614..3744111 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FNP80_RS20035 3744287..3745036 - 750 WP_024192679.1 C40 family peptidase -
FNP80_RS20040 3745246..3745506 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FNP80_RS20045 3745509..3745787 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FNP80_RS20050 3745943..3746683 + 741 WP_001225679.1 murein L,D-transpeptidase -
FNP80_RS20055 3746654..3747421 - 768 WP_000333380.1 class II glutamine amidotransferase -
FNP80_RS20060 3747627..3748205 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T128640 WP_000615983.1 NZ_CP041442:3745509-3745787 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128640 NZ_CP041442:3745509-3745787 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCATCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT128640 WP_000729703.1 NZ_CP041442:3745246-3745506 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128640 NZ_CP041442:3745246-3745506 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References