Detailed information of TA system TA128639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3739049..3739743
• Replicon: chromosome
• Accession: NZ_CP041442
• Organism: Escherichia coli strain YPE12

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FNP80_RS20005
• Protein ID: WP_001263493.1
• Coordinates: 3739049..3739447 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FNP80_RS20010
• Protein ID: WP_000554757.1
• Coordinates: 3739450..3739743 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP80_RS19975	3734608..3735066	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FNP80_RS19980	3735327..3736784	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FNP80_RS19985	3736841..3737362	-	522	Protein_3556	peptide chain release factor H	-
FNP80_RS19990	3737361..3737564	-	204	Protein_3557	RNA ligase RtcB family protein	-
FNP80_RS19995	3737810..3738262	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FNP80_RS20000	3738336..3739033	+	698	Protein_3559	IS1 family transposase	-
FNP80_RS20005	3739049..3739447	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FNP80_RS20010	3739450..3739743	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FNP80_RS20015	3739795..3740850	-	1056	WP_001226164.1	DNA polymerase IV	-
FNP80_RS20020	3740921..3741706	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
FNP80_RS20025	3741651..3743390	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
FNP80_RS20030	3743614..3744111	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3652484..3744913	92429
	-	flank	IS/Tn	-	-	3738740..3739033	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T128639 WP_001263493.1 NZ_CP041442:c3739447-3739049 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T128639 NZ_CP041442:c3739447-3739049 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT128639 WP_000554757.1 NZ_CP041442:c3739743-3739450 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT128639 NZ_CP041442:c3739743-3739450 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6