Detailed information of TA system TA128618

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75968..76232
• Replicon: plasmid pYPE12-122k
• Accession: NZ_CP041441
• Organism: Escherichia coli strain YPE12

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: FNP80_RS01325
• Protein ID: WP_001303307.1
• Coordinates: 76080..76232 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	75968..76030 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP80_RS01310	72070..73140	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
FNP80_RS01315	73159..74367	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	74547..74607	-	61	NuclAT_1	-	-
-	74547..74607	-	61	NuclAT_1	-	-
-	74547..74607	-	61	NuclAT_1	-	-
-	74547..74607	-	61	NuclAT_1	-	-
FNP80_RS01320	74674..75765	-	1092	WP_000426061.1	hypothetical protein	-
-	75968..76030	-	63	NuclAT_0	-	Antitoxin
-	75968..76030	-	63	NuclAT_0	-	Antitoxin
-	75968..76030	-	63	NuclAT_0	-	Antitoxin
-	75968..76030	-	63	NuclAT_0	-	Antitoxin
FNP80_RS01325	76080..76232	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
FNP80_RS01330	76304..76555	-	252	WP_001291968.1	hypothetical protein	-
-	76942..76993	-	52	NuclAT_2	-	-
-	76942..76993	-	52	NuclAT_2	-	-
-	76942..76993	-	52	NuclAT_2	-	-
-	76942..76993	-	52	NuclAT_2	-	-
FNP80_RS25370	77479..77655	-	177	WP_001054900.1	hypothetical protein	-
FNP80_RS01345	77864..78073	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
FNP80_RS01350	78171..78785	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
FNP80_RS01355	78861..81029	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / tet(M) / mph(A) / aac(3)-IId / erm(B)	-	1..122347	122347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T128618 WP_001303307.1 NZ_CP041441:76080-76232 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T128618 NZ_CP041441:76080-76232 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT128618 NZ_CP041441:c76030-75968 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin