Detailed information of TA system TA128610

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14752..15021
• Replicon: plasmid pYPE12-106k
• Accession: NZ_CP041439
• Organism: Escherichia coli strain YPE12

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FNP80_RS00125
• Protein ID: WP_001312861.1
• Coordinates: 14863..15021 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	14752..14817 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP80_RS00100	10465..10992	+	528	WP_151140338.1	single-stranded DNA-binding protein	-
FNP80_RS00105	11050..11282	+	233	Protein_13	DUF905 family protein	-
FNP80_RS00110	11343..13364	+	2022	Protein_14	ParB/RepB/Spo0J family partition protein	-
FNP80_RS00115	13433..13867	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FNP80_RS00120	13864..14583	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	14595..14819	+	225	NuclAT_0	-	-
-	14595..14819	+	225	NuclAT_0	-	-
-	14595..14819	+	225	NuclAT_0	-	-
-	14595..14819	+	225	NuclAT_0	-	-
FNP80_RS25330	14604..14783	-	180	WP_001309233.1	hypothetical protein	-
-	14752..14817	+	66	-	-	Antitoxin
FNP80_RS00125	14863..15021	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FNP80_RS25335	15259..15636	-	378	Protein_19	hypothetical protein	-
FNP80_RS00145	15936..16232	+	297	WP_001272251.1	hypothetical protein	-
FNP80_RS00150	16343..17164	+	822	WP_151140339.1	DUF945 domain-containing protein	-
FNP80_RS00155	17461..18108	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
FNP80_RS00160	18385..18768	+	384	WP_000124981.1	relaxosome protein TraM	-
FNP80_RS00165	18959..19645	+	687	WP_015059009.1	PAS domain-containing protein	-
FNP80_RS00170	19739..19966	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / blaTEM-1B / rmtB	-	1..106630	106630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T128610 WP_001312861.1 NZ_CP041439:14863-15021 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128610 NZ_CP041439:14863-15021 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT128610 NZ_CP041439:14752-14817 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin