Detailed information of TA system TA128605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4732854..4733456
• Replicon: chromosome
• Accession: NZ_CP041437
• Organism: Escherichia coli strain STEC005

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FNE80_RS22925
• Protein ID: WP_000897305.1
• Coordinates: 4733145..4733456 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FNE80_RS22920
• Protein ID: WP_000356397.1
• Coordinates: 4732854..4733144 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE80_RS22895	4728780..4729682	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FNE80_RS22900	4729679..4730314	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FNE80_RS22905	4730311..4731240	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FNE80_RS22910	4731570..4731812	-	243	WP_001086388.1	hypothetical protein	-
FNE80_RS22915	4732031..4732249	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FNE80_RS22920	4732854..4733144	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FNE80_RS22925	4733145..4733456	-	312	WP_000897305.1	hypothetical protein	Toxin
FNE80_RS22930	4733685..4734593	+	909	WP_001162704.1	alpha/beta hydrolase	-
FNE80_RS22935	4734657..4735598	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FNE80_RS22940	4735643..4736080	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FNE80_RS22945	4736077..4736949	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FNE80_RS22950	4736943..4737542	-	600	WP_001295269.1	glucose-1-phosphatase	-
FNE80_RS22955	4737641..4738426	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T128605 WP_000897305.1 NZ_CP041437:c4733456-4733145 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128605 NZ_CP041437:c4733456-4733145 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128605 WP_000356397.1 NZ_CP041437:c4733144-4732854 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128605 NZ_CP041437:c4733144-4732854 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7