Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1850682..1851184 Replicon chromosome
Accession NZ_CP041437
Organism Escherichia coli strain STEC005

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FNE80_RS08730 Protein ID WP_000767829.1
Coordinates 1850930..1851184 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNE80_RS08725 Protein ID WP_001259255.1
Coordinates 1850682..1850933 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE80_RS08700 1845860..1846927 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FNE80_RS08705 1846927..1847997 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FNE80_RS08710 1847994..1849298 - 1305 WP_001317939.1 histidinol dehydrogenase -
FNE80_RS08715 1849304..1850203 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FNE80_RS08720 1850349..1850399 - 51 WP_001364200.1 his operon leader peptide -
FNE80_RS08725 1850682..1850933 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNE80_RS08730 1850930..1851184 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNE80_RS08735 1851267..1852091 + 825 WP_000754763.1 SDR family oxidoreductase -
FNE80_RS08740 1852137..1853066 + 930 WP_000803351.1 LysR family transcriptional regulator -
FNE80_RS08745 1853281..1853343 + 63 WP_010723108.1 membrane protein YoeI -
FNE80_RS08750 1853333..1854691 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNE80_RS08755 1854870..1855928 + 1059 WP_000492335.1 transport protein YeeE -
FNE80_RS08760 1855942..1856169 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128590 WP_000767829.1 NZ_CP041437:1850930-1851184 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128590 NZ_CP041437:1850930-1851184 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128590 WP_001259255.1 NZ_CP041437:1850682-1850933 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128590 NZ_CP041437:1850682-1850933 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References