Detailed information of TA system TA128581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4710925..4711527
• Replicon: chromosome
• Accession: NZ_CP041435
• Organism: Escherichia coli strain STEC309

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FNE81_RS22920
• Protein ID: WP_000897305.1
• Coordinates: 4711216..4711527 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FNE81_RS22915
• Protein ID: WP_000356397.1
• Coordinates: 4710925..4711215 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE81_RS22890	4706850..4707752	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FNE81_RS22895	4707749..4708384	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FNE81_RS22900	4708381..4709310	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FNE81_RS22905	4709640..4709882	-	243	WP_001087409.1	hypothetical protein	-
FNE81_RS22910	4710102..4710320	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
FNE81_RS22915	4710925..4711215	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FNE81_RS22920	4711216..4711527	-	312	WP_000897305.1	hypothetical protein	Toxin
FNE81_RS22925	4711756..4712664	+	909	WP_001162704.1	alpha/beta hydrolase	-
FNE81_RS22930	4712728..4713669	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FNE81_RS22935	4713714..4714151	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FNE81_RS22940	4714148..4715020	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FNE81_RS22945	4715014..4715613	-	600	WP_001295269.1	glucose-1-phosphatase	-
FNE81_RS22950	4715712..4716497	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T128581 WP_000897305.1 NZ_CP041435:c4711527-4711216 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128581 NZ_CP041435:c4711527-4711216 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128581 WP_000356397.1 NZ_CP041435:c4711215-4710925 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128581 NZ_CP041435:c4711215-4710925 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7