Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3846510..3847051 Replicon chromosome
Accession NZ_CP041435
Organism Escherichia coli strain STEC309

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FNE81_RS18835 Protein ID WP_000615976.1
Coordinates 3846773..3847051 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FNE81_RS18830 Protein ID WP_000729704.1
Coordinates 3846510..3846770 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE81_RS18805 3842293..3843078 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
FNE81_RS18810 3843050..3844762 + 1713 Protein_3684 flagellar biosynthesis protein FlhA -
FNE81_RS18815 3844867..3845145 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FNE81_RS18820 3845138..3845494 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
FNE81_RS18825 3845551..3846324 - 774 WP_000543895.1 C40 family peptidase -
FNE81_RS18830 3846510..3846770 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FNE81_RS18835 3846773..3847051 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FNE81_RS18840 3847207..3847947 + 741 WP_001225679.1 murein L,D-transpeptidase -
FNE81_RS18845 3847918..3848685 - 768 WP_000333380.1 class II glutamine amidotransferase -
FNE81_RS18850 3848891..3849469 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T128576 WP_000615976.1 NZ_CP041435:3846773-3847051 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128576 NZ_CP041435:3846773-3847051 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT128576 WP_000729704.1 NZ_CP041435:3846510-3846770 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128576 NZ_CP041435:3846510-3846770 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References