Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1874265..1874767 Replicon chromosome
Accession NZ_CP041435
Organism Escherichia coli strain STEC309

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FNE81_RS08865 Protein ID WP_000767829.1
Coordinates 1874513..1874767 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNE81_RS08860 Protein ID WP_001259255.1
Coordinates 1874265..1874516 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE81_RS08835 1869443..1870510 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FNE81_RS08840 1870510..1871580 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
FNE81_RS08845 1871577..1872881 - 1305 WP_148460516.1 histidinol dehydrogenase -
FNE81_RS08850 1872887..1873786 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
FNE81_RS08855 1873932..1873982 - 51 WP_001364200.1 his operon leader peptide -
FNE81_RS08860 1874265..1874516 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNE81_RS08865 1874513..1874767 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNE81_RS08870 1874850..1875674 + 825 WP_000754749.1 SDR family oxidoreductase -
FNE81_RS08875 1875720..1876649 + 930 WP_000803351.1 LysR family transcriptional regulator -
FNE81_RS08880 1876864..1876926 + 63 WP_010723108.1 membrane protein YoeI -
FNE81_RS08885 1876916..1878274 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNE81_RS08890 1878453..1879511 + 1059 WP_148460515.1 transport protein YeeE -
FNE81_RS08895 1879525..1879752 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128562 WP_000767829.1 NZ_CP041435:1874513-1874767 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128562 NZ_CP041435:1874513-1874767 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128562 WP_001259255.1 NZ_CP041435:1874265-1874516 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128562 NZ_CP041435:1874265-1874516 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References