Detailed information of TA system TA128552

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52323..52749
• Replicon: plasmid pSTEC313
• Accession: NZ_CP041434
• Organism: Escherichia coli strain STEC313

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FNE82_RS25145
• Protein ID: WP_001312861.1
• Coordinates: 52323..52481 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52525..52749 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE82_RS25110	47375..47602	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FNE82_RS25115	47696..48382	-	687	WP_000332492.1	PAS domain-containing protein	-
FNE82_RS25120	48572..48955	-	384	WP_000124981.1	relaxosome protein TraM	-
FNE82_RS25125	49232..49879	+	648	WP_123007297.1	transglycosylase SLT domain-containing protein	-
FNE82_RS25130	50176..50997	-	822	WP_074014913.1	DUF945 domain-containing protein	-
FNE82_RS25135	51115..51312	-	198	WP_052318758.1	hypothetical protein	-
FNE82_RS25500	51873..52028	-	156	WP_170938106.1	hypothetical protein	-
FNE82_RS25145	52323..52481	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	52525..52749	-	225	NuclAT_0	-	Antitoxin
-	52525..52749	-	225	NuclAT_0	-	Antitoxin
-	52525..52749	-	225	NuclAT_0	-	Antitoxin
-	52525..52749	-	225	NuclAT_0	-	Antitoxin
FNE82_RS25150	52561..52749	+	189	WP_001299721.1	hypothetical protein	-
FNE82_RS25155	52761..53480	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
FNE82_RS25160	53477..53911	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FNE82_RS25165	53966..55924	-	1959	WP_161620594.1	ParB/RepB/Spo0J family partition protein	-
FNE82_RS25170	55990..56223	-	234	WP_000005990.1	DUF905 family protein	-
FNE82_RS25175	56286..56825	-	540	WP_161620593.1	single-stranded DNA-binding protein	-
FNE82_RS25505	56851..57057	-	207	WP_000275853.1	hypothetical protein	-
FNE82_RS25185	57298..57552	-	255	WP_135460463.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD	1..80039	80039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T128552 WP_001312861.1 NZ_CP041434:c52481-52323 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128552 NZ_CP041434:c52481-52323 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT128552 NZ_CP041434:c52749-52525 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin