Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2916159..2916700 Replicon chromosome
Accession NZ_CP041433
Organism Escherichia coli strain STEC313

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag FNE82_RS14210 Protein ID WP_000615979.1
Coordinates 2916159..2916437 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FNE82_RS14215 Protein ID WP_000729704.1
Coordinates 2916440..2916700 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE82_RS14195 2913741..2914319 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FNE82_RS14200 2914525..2915292 + 768 WP_000333380.1 class II glutamine amidotransferase -
FNE82_RS14205 2915263..2916003 - 741 WP_001225679.1 murein L,D-transpeptidase -
FNE82_RS14210 2916159..2916437 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FNE82_RS14215 2916440..2916700 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FNE82_RS14220 2916886..2917659 + 774 WP_000543899.1 C40 family peptidase -
FNE82_RS14225 2917716..2918072 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FNE82_RS14230 2918065..2918343 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FNE82_RS14235 2918448..2920160 - 1713 Protein_2785 flagellar biosynthesis protein FlhA -
FNE82_RS14240 2920132..2920917 + 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T128531 WP_000615979.1 NZ_CP041433:c2916437-2916159 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128531 NZ_CP041433:c2916437-2916159 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT128531 WP_000729704.1 NZ_CP041433:c2916700-2916440 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128531 NZ_CP041433:c2916700-2916440 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References