Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1904565..1905067 Replicon chromosome
Accession NZ_CP041433
Organism Escherichia coli strain STEC313

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FNE82_RS09125 Protein ID WP_000767829.1
Coordinates 1904813..1905067 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNE82_RS09120 Protein ID WP_001259255.1
Coordinates 1904565..1904816 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE82_RS09095 1899743..1900810 - 1068 WP_001594218.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FNE82_RS09100 1900810..1901880 - 1071 WP_001594217.1 histidinol-phosphate transaminase -
FNE82_RS09105 1901877..1903181 - 1305 WP_001594215.1 histidinol dehydrogenase -
FNE82_RS09110 1903187..1904086 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FNE82_RS09115 1904232..1904282 - 51 WP_001364200.1 his operon leader peptide -
FNE82_RS09120 1904565..1904816 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNE82_RS09125 1904813..1905067 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNE82_RS09130 1905150..1905974 + 825 WP_000754737.1 SDR family oxidoreductase -
FNE82_RS09135 1906020..1906949 + 930 WP_001374016.1 LysR family transcriptional regulator -
FNE82_RS09140 1907164..1907226 + 63 WP_010723108.1 membrane protein YoeI -
FNE82_RS09145 1907216..1908574 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNE82_RS09150 1908753..1909811 + 1059 WP_000492324.1 transport protein YeeE -
FNE82_RS09155 1909825..1910052 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128524 WP_000767829.1 NZ_CP041433:1904813-1905067 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128524 NZ_CP041433:1904813-1905067 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128524 WP_001259255.1 NZ_CP041433:1904565-1904816 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128524 NZ_CP041433:1904565-1904816 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References