Detailed information of TA system TA128513

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36607..37033
• Replicon: plasmid pSTEC316
• Accession: NZ_CP041432
• Organism: Escherichia coli strain STEC316

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FNE83_RS26095
• Protein ID: WP_001312861.1
• Coordinates: 36607..36765 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36809..37033 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE83_RS26060	31659..31886	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FNE83_RS26065	31980..32666	-	687	WP_000332492.1	PAS domain-containing protein	-
FNE83_RS26070	32856..33239	-	384	WP_000124981.1	relaxosome protein TraM	-
FNE83_RS26075	33516..34163	+	648	WP_123007297.1	transglycosylase SLT domain-containing protein	-
FNE83_RS26080	34460..35281	-	822	WP_074014913.1	DUF945 domain-containing protein	-
FNE83_RS26085	35399..35596	-	198	WP_052318758.1	hypothetical protein	-
FNE83_RS26590	36157..36312	-	156	WP_170938106.1	hypothetical protein	-
FNE83_RS26095	36607..36765	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	36809..37033	-	225	NuclAT_0	-	Antitoxin
-	36809..37033	-	225	NuclAT_0	-	Antitoxin
-	36809..37033	-	225	NuclAT_0	-	Antitoxin
-	36809..37033	-	225	NuclAT_0	-	Antitoxin
FNE83_RS26100	36845..37033	+	189	WP_001299721.1	hypothetical protein	-
FNE83_RS26105	37045..37764	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
FNE83_RS26110	37761..38195	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FNE83_RS26115	38250..40208	-	1959	WP_161620594.1	ParB/RepB/Spo0J family partition protein	-
FNE83_RS26120	40274..40507	-	234	WP_000005990.1	DUF905 family protein	-
FNE83_RS26125	40570..41109	-	540	WP_161620593.1	single-stranded DNA-binding protein	-
FNE83_RS26595	41135..41341	-	207	WP_000275853.1	hypothetical protein	-
FNE83_RS26135	41582..41830	-	249	WP_074430098.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..80029	80029

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T128513 WP_001312861.1 NZ_CP041432:c36765-36607 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128513 NZ_CP041432:c36765-36607 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT128513 NZ_CP041432:c37033-36809 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin