Detailed information of TA system TA128511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5041567..5042169
• Replicon: chromosome
• Accession: NZ_CP041431
• Organism: Escherichia coli strain STEC316

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FNE83_RS24915
• Protein ID: WP_000897305.1
• Coordinates: 5041858..5042169 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FNE83_RS24910
• Protein ID: WP_000356397.1
• Coordinates: 5041567..5041857 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE83_RS24885	5037493..5038395	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FNE83_RS24890	5038392..5039027	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FNE83_RS24895	5039024..5039953	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FNE83_RS24900	5040283..5040525	-	243	WP_001087409.1	hypothetical protein	-
FNE83_RS24905	5040744..5040962	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FNE83_RS24910	5041567..5041857	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FNE83_RS24915	5041858..5042169	-	312	WP_000897305.1	hypothetical protein	Toxin
FNE83_RS24920	5042398..5043306	+	909	WP_001299484.1	alpha/beta hydrolase	-
FNE83_RS24925	5043370..5044311	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
FNE83_RS24930	5044356..5044793	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FNE83_RS24935	5044790..5045662	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FNE83_RS24940	5045656..5046255	-	600	WP_001295269.1	glucose-1-phosphatase	-
FNE83_RS24945	5046354..5047139	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T128511 WP_000897305.1 NZ_CP041431:c5042169-5041858 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128511 NZ_CP041431:c5042169-5041858 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128511 WP_000356397.1 NZ_CP041431:c5041857-5041567 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128511 NZ_CP041431:c5041857-5041567 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7