Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2934828..2935330 Replicon chromosome
Accession NZ_CP041431
Organism Escherichia coli strain STEC316

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FNE83_RS14795 Protein ID WP_000767829.1
Coordinates 2934828..2935082 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNE83_RS14800 Protein ID WP_001259255.1
Coordinates 2935079..2935330 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE83_RS14765 2929843..2930070 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
FNE83_RS14770 2930084..2931142 - 1059 WP_000492324.1 transport protein YeeE -
FNE83_RS14775 2931321..2932679 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNE83_RS14780 2932669..2932731 - 63 WP_010723108.1 membrane protein YoeI -
FNE83_RS14785 2932946..2933875 - 930 WP_001374016.1 LysR family transcriptional regulator -
FNE83_RS14790 2933921..2934745 - 825 WP_000754737.1 SDR family oxidoreductase -
FNE83_RS14795 2934828..2935082 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNE83_RS14800 2935079..2935330 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNE83_RS14805 2935613..2935663 + 51 WP_001364200.1 his operon leader peptide -
FNE83_RS14810 2935809..2936708 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FNE83_RS14815 2936714..2938018 + 1305 WP_001594215.1 histidinol dehydrogenase -
FNE83_RS14820 2938015..2939085 + 1071 WP_001594217.1 histidinol-phosphate transaminase -
FNE83_RS14825 2939085..2940152 + 1068 WP_001594218.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128501 WP_000767829.1 NZ_CP041431:c2935082-2934828 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128501 NZ_CP041431:c2935082-2934828 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128501 WP_001259255.1 NZ_CP041431:c2935330-2935079 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128501 NZ_CP041431:c2935330-2935079 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References