Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 652137..652936 Replicon chromosome
Accession NZ_CP041431
Organism Escherichia coli strain STEC316

Toxin (Protein)


Gene name yhaV Uniprot ID A0A6H2GMC5
Locus tag FNE83_RS03230 Protein ID WP_000347279.1
Coordinates 652137..652601 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FNE83_RS03235 Protein ID WP_001307405.1
Coordinates 652601..652936 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE83_RS03205 647590..648468 - 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
FNE83_RS03210 648458..649237 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
FNE83_RS03215 649248..649721 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
FNE83_RS03220 649744..651024 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
FNE83_RS03225 651273..652082 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FNE83_RS03230 652137..652601 - 465 WP_000347279.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FNE83_RS03235 652601..652936 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FNE83_RS03240 653085..654656 - 1572 WP_001273741.1 galactarate dehydratase -
FNE83_RS03245 655031..656365 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FNE83_RS03250 656381..657151 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17869.24 Da        Isoelectric Point: 9.4947

>T128480 WP_000347279.1 NZ_CP041431:c652601-652137 [Escherichia coli]
MDFPQRVNGWVLYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Download         Length: 465 bp

>T128480 NZ_CP041431:c652601-652137 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGTGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCTTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT128480 WP_001307405.1 NZ_CP041431:c652936-652601 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT128480 NZ_CP041431:c652936-652601 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GMC5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References