Detailed information of TA system TA128475

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 77023..77449
• Replicon: plasmid pSTEC367
• Accession: NZ_CP041430
• Organism: Escherichia coli strain STEC367

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FNE84_RS25260
• Protein ID: WP_001312861.1
• Coordinates: 77023..77181 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	77225..77449 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE84_RS25225	72075..72302	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FNE84_RS25230	72396..73082	-	687	WP_000332492.1	PAS domain-containing protein	-
FNE84_RS25235	73272..73655	-	384	WP_000124981.1	relaxosome protein TraM	-
FNE84_RS25240	73932..74579	+	648	WP_123007297.1	transglycosylase SLT domain-containing protein	-
FNE84_RS25245	74876..75697	-	822	WP_074014913.1	DUF945 domain-containing protein	-
FNE84_RS25250	75815..76012	-	198	WP_052318758.1	hypothetical protein	-
FNE84_RS25440	76573..76728	-	156	WP_170938106.1	hypothetical protein	-
FNE84_RS25260	77023..77181	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	77225..77449	-	225	NuclAT_0	-	Antitoxin
-	77225..77449	-	225	NuclAT_0	-	Antitoxin
-	77225..77449	-	225	NuclAT_0	-	Antitoxin
-	77225..77449	-	225	NuclAT_0	-	Antitoxin
FNE84_RS25265	77261..77449	+	189	WP_001299721.1	hypothetical protein	-
FNE84_RS25270	77461..78180	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
FNE84_RS25275	78177..78611	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..80039	80039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T128475 WP_001312861.1 NZ_CP041430:c77181-77023 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128475 NZ_CP041430:c77181-77023 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT128475 NZ_CP041430:c77449-77225 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin