Detailed information of TA system TA128458

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4041732..4042103
• Replicon: chromosome
• Accession: NZ_CP041429
• Organism: Escherichia coli strain STEC367

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: Q8Z5Y6
• Locus tag: FNE84_RS19635
• Protein ID: WP_010989194.1
• Coordinates: 4041909..4042103 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4041732..4041910 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE84_RS19605	4037484..4037657	+	174	WP_001296046.1	hypothetical protein	-
FNE84_RS19610	4037687..4039060	+	1374	WP_000123738.1	ATP-dependent RNA helicase DbpA	-
FNE84_RS19615	4039189..4040124	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FNE84_RS19620	4040176..4041411	-	1236	WP_071998909.1	site-specific integrase	-
FNE84_RS19625	4041413..4041628	-	216	WP_000079604.1	excisionase XisR	-
FNE84_RS19630	4041728..4041916	-	189	WP_001383994.1	DUF1187 family protein	-
-	4041732..4041910	+	179	NuclAT_0	-	Antitoxin
-	4041732..4041910	+	179	NuclAT_0	-	Antitoxin
-	4041732..4041910	+	179	NuclAT_0	-	Antitoxin
-	4041732..4041910	+	179	NuclAT_0	-	Antitoxin
FNE84_RS19635	4041909..4042103	-	195	WP_010989194.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FNE84_RS19640	4042167..4043219	-	1053	WP_001774814.1	recombinase RecT	-
FNE84_RS19645	4043231..4046380	-	3150	WP_032221030.1	exodeoxyribonuclease VIII	-
FNE84_RS19650	4046404..4046700	-	297	WP_000022247.1	hypothetical protein	-
FNE84_RS19655	4046775..4046945	-	171	WP_077633604.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4040176..4090176	50000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7077.01 Da
• Isoelectric Point: 9.2886

>T128458 WP_010989194.1 NZ_CP041429:c4042103-4041909 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T128458 NZ_CP041429:c4042103-4041909 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGTCCGTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCAAAGTGTAACGGATGCGAATCCCGAACTGGCTATAGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT128458 NZ_CP041429:4041732-4041910 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCAGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A716R3V7

Antitoxin