Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2869103..2869644 Replicon chromosome
Accession NZ_CP041429
Organism Escherichia coli strain STEC367

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag FNE84_RS13895 Protein ID WP_000615979.1
Coordinates 2869103..2869381 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FNE84_RS13900 Protein ID WP_000729704.1
Coordinates 2869384..2869644 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE84_RS13880 2866685..2867263 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FNE84_RS13885 2867469..2868236 + 768 WP_000333380.1 class II glutamine amidotransferase -
FNE84_RS13890 2868207..2868947 - 741 WP_001225679.1 murein L,D-transpeptidase -
FNE84_RS13895 2869103..2869381 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FNE84_RS13900 2869384..2869644 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FNE84_RS13905 2869830..2870603 + 774 WP_000543899.1 C40 family peptidase -
FNE84_RS13910 2870660..2871016 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FNE84_RS13915 2871009..2871287 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FNE84_RS13920 2871392..2873104 - 1713 Protein_2721 flagellar biosynthesis protein FlhA -
FNE84_RS13925 2873076..2873861 + 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T128449 WP_000615979.1 NZ_CP041429:c2869381-2869103 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128449 NZ_CP041429:c2869381-2869103 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT128449 WP_000729704.1 NZ_CP041429:c2869644-2869384 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128449 NZ_CP041429:c2869644-2869384 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References