Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1977075..1977577 Replicon chromosome
Accession NZ_CP041429
Organism Escherichia coli strain STEC367

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FNE84_RS09415 Protein ID WP_000767829.1
Coordinates 1977323..1977577 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNE84_RS09410 Protein ID WP_001259255.1
Coordinates 1977075..1977326 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE84_RS09385 1972253..1973320 - 1068 WP_001594218.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FNE84_RS09390 1973320..1974390 - 1071 WP_001594217.1 histidinol-phosphate transaminase -
FNE84_RS09395 1974387..1975691 - 1305 WP_001594215.1 histidinol dehydrogenase -
FNE84_RS09400 1975697..1976596 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FNE84_RS09405 1976742..1976792 - 51 WP_001364200.1 his operon leader peptide -
FNE84_RS09410 1977075..1977326 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNE84_RS09415 1977323..1977577 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNE84_RS09420 1977660..1978484 + 825 WP_000754737.1 SDR family oxidoreductase -
FNE84_RS09425 1978530..1979459 + 930 WP_001374016.1 LysR family transcriptional regulator -
FNE84_RS09430 1979674..1979736 + 63 WP_010723108.1 membrane protein YoeI -
FNE84_RS09435 1979726..1981084 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNE84_RS09440 1981263..1982321 + 1059 WP_000492324.1 transport protein YeeE -
FNE84_RS09445 1982335..1982562 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128443 WP_000767829.1 NZ_CP041429:1977323-1977577 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128443 NZ_CP041429:1977323-1977577 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128443 WP_001259255.1 NZ_CP041429:1977075-1977326 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128443 NZ_CP041429:1977075-1977326 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References