Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1828924..1829426 Replicon chromosome
Accession NZ_CP041425
Organism Escherichia coli strain STEC388

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FNE85_RS08780 Protein ID WP_000767829.1
Coordinates 1829172..1829426 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNE85_RS08775 Protein ID WP_001259255.1
Coordinates 1828924..1829175 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE85_RS08750 1824102..1825169 - 1068 WP_161623626.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FNE85_RS08755 1825169..1826239 - 1071 WP_000108940.1 histidinol-phosphate transaminase -
FNE85_RS08760 1826236..1827540 - 1305 WP_001317939.1 histidinol dehydrogenase -
FNE85_RS08765 1827546..1828445 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FNE85_RS08770 1828591..1828641 - 51 WP_001364200.1 his operon leader peptide -
FNE85_RS08775 1828924..1829175 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNE85_RS08780 1829172..1829426 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNE85_RS08785 1829509..1830333 + 825 WP_096128835.1 SDR family oxidoreductase -
FNE85_RS08790 1830379..1831308 + 930 WP_000803351.1 LysR family transcriptional regulator -
FNE85_RS08795 1831523..1831585 + 63 WP_010723108.1 membrane protein YoeI -
FNE85_RS08800 1831575..1832933 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNE85_RS08805 1833112..1834170 + 1059 WP_096128836.1 transport protein YeeE -
FNE85_RS08810 1834184..1834411 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128417 WP_000767829.1 NZ_CP041425:1829172-1829426 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128417 NZ_CP041425:1829172-1829426 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128417 WP_001259255.1 NZ_CP041425:1828924-1829175 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128417 NZ_CP041425:1828924-1829175 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References