Detailed information of TA system TA128416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1106498..1107225
• Replicon: chromosome
• Accession: NZ_CP041425
• Organism: Escherichia coli strain STEC388

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: FNE85_RS05390
• Protein ID: WP_000547564.1
• Coordinates: 1106498..1106809 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FNE85_RS05395
• Protein ID: WP_000126294.1
• Coordinates: 1106806..1107225 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE85_RS05360	1101640..1103349	+	1710	WP_096128740.1	formate hydrogenlyase subunit HycE	-
FNE85_RS05365	1103359..1103901	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
FNE85_RS05370	1103901..1104668	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
FNE85_RS05375	1104665..1105075	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
FNE85_RS05380	1105068..1105538	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FNE85_RS05385	1105563..1106324	+	762	WP_001026446.1	hypothetical protein	-
FNE85_RS05390	1106498..1106809	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
FNE85_RS05395	1106806..1107225	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FNE85_RS05400	1107339..1108763	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
FNE85_RS05405	1108772..1110229	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FNE85_RS05410	1110489..1111499	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
FNE85_RS05415	1111648..1112175	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T128416 WP_000547564.1 NZ_CP041425:1106498-1106809 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T128416 NZ_CP041425:1106498-1106809 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT128416 WP_000126294.1 NZ_CP041425:1106806-1107225 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT128416 NZ_CP041425:1106806-1107225 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5