Detailed information of TA system TA128406

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64292..64562
• Replicon: plasmid pSTEC409_2
• Accession: NZ_CP041424
• Organism: Escherichia coli strain STEC409

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FNE86_RS23760
• Protein ID: WP_001312861.1
• Coordinates: 64404..64562 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	64292..64355 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE86_RS23975	59432..59587	+	156	WP_170938106.1	hypothetical protein	-
FNE86_RS23980	59580..59915	+	336	WP_077763704.1	hypothetical protein	-
FNE86_RS23985	59828..60034	+	207	WP_000275853.1	hypothetical protein	-
FNE86_RS23730	60060..60599	+	540	WP_161620593.1	single-stranded DNA-binding protein	-
FNE86_RS23735	60662..60895	+	234	WP_000005990.1	DUF905 family protein	-
FNE86_RS23740	60961..62919	+	1959	WP_161620594.1	ParB/RepB/Spo0J family partition protein	-
FNE86_RS23745	62974..63408	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FNE86_RS23750	63405..64124	+	720	WP_161620595.1	plasmid SOS inhibition protein A	-
FNE86_RS23755	64136..64324	-	189	WP_001299721.1	hypothetical protein	-
-	64136..64360	+	225	NuclAT_0	-	-
-	64136..64360	+	225	NuclAT_0	-	-
-	64136..64360	+	225	NuclAT_0	-	-
-	64136..64360	+	225	NuclAT_0	-	-
-	64292..64355	-	64	-	-	Antitoxin
FNE86_RS23760	64404..64562	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FNE86_RS23990	64857..65012	+	156	WP_170938106.1	hypothetical protein	-
FNE86_RS23770	65573..65770	+	198	WP_052318758.1	hypothetical protein	-
FNE86_RS23775	65888..66709	+	822	WP_074014913.1	DUF945 domain-containing protein	-
FNE86_RS23780	67006..67608	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
FNE86_RS23785	67929..68312	+	384	WP_001151524.1	relaxosome protein TraM	-
FNE86_RS23790	68499..69188	+	690	WP_021532962.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..81110	81110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T128406 WP_001312861.1 NZ_CP041424:64404-64562 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128406 NZ_CP041424:64404-64562 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT128406 NZ_CP041424:c64355-64292 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin