Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3285072..3285574 Replicon chromosome
Accession NZ_CP041422
Organism Escherichia coli strain STEC409

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag FNE86_RS16260 Protein ID WP_001471733.1
Coordinates 3285072..3285326 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNE86_RS16265 Protein ID WP_001259255.1
Coordinates 3285323..3285574 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE86_RS16240 3281565..3282923 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNE86_RS16245 3282913..3282975 - 63 WP_010723108.1 membrane protein YoeI -
FNE86_RS16250 3283190..3284119 - 930 WP_001374016.1 LysR family transcriptional regulator -
FNE86_RS16255 3284165..3284989 - 825 WP_000754737.1 SDR family oxidoreductase -
FNE86_RS16260 3285072..3285326 - 255 WP_001471733.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNE86_RS16265 3285323..3285574 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNE86_RS16270 3285857..3285907 + 51 WP_001364200.1 his operon leader peptide -
FNE86_RS16275 3286053..3286952 + 900 WP_001374031.1 ATP phosphoribosyltransferase -
FNE86_RS16280 3286958..3288262 + 1305 WP_001748534.1 histidinol dehydrogenase -
FNE86_RS16285 3288259..3289329 + 1071 WP_001374029.1 histidinol-phosphate transaminase -
FNE86_RS16290 3289329..3290396 + 1068 WP_001374028.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T128400 WP_001471733.1 NZ_CP041422:c3285326-3285072 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDIRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128400 NZ_CP041422:c3285326-3285072 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATATCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128400 WP_001259255.1 NZ_CP041422:c3285574-3285323 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128400 NZ_CP041422:c3285574-3285323 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References