Detailed information of TA system TA128388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1543142..1543836
• Replicon: chromosome
• Accession: NZ_CP041422
• Organism: Escherichia coli strain STEC409

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FNE86_RS07575
• Protein ID: WP_001263493.1
• Coordinates: 1543438..1543836 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FNE86_RS07570
• Protein ID: WP_000554757.1
• Coordinates: 1543142..1543435 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE86_RS07545	1538959..1539426	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
FNE86_RS07550	1539446..1540162	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
FNE86_RS07555	1540175..1541038	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
FNE86_RS07560	1541041..1541964	+	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
FNE86_RS07565	1542035..1543090	+	1056	WP_001226164.1	DNA polymerase IV	-
FNE86_RS07570	1543142..1543435	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FNE86_RS07575	1543438..1543836	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FNE86_RS07580	1543846..1544298	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FNE86_RS07585	1544544..1544747	+	204	Protein_1474	RNA ligase RtcB family protein	-
FNE86_RS07590	1544746..1545267	+	522	Protein_1475	peptide chain release factor H	-
FNE86_RS07595	1545324..1546781	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FNE86_RS07600	1547042..1547500	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FNE86_RS07605	1547592..1548836	+	1245	WP_000189541.1	esterase FrsA	-
-	1548096..1548176	+	81	NuclAT_7	-	-
-	1548096..1548176	+	81	NuclAT_7	-	-
-	1548096..1548176	+	81	NuclAT_7	-	-
-	1548096..1548176	+	81	NuclAT_7	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T128388 WP_001263493.1 NZ_CP041422:1543438-1543836 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T128388 NZ_CP041422:1543438-1543836 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT128388 WP_000554757.1 NZ_CP041422:1543142-1543435 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT128388 NZ_CP041422:1543142-1543435 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6