Detailed information of TA system TA128375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4505131..4505733
• Replicon: chromosome
• Accession: NZ_CP041416
• Organism: Escherichia coli strain STEC711

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FNE87_RS21685
• Protein ID: WP_000897305.1
• Coordinates: 4505422..4505733 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FNE87_RS21680
• Protein ID: WP_000356397.1
• Coordinates: 4505131..4505421 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE87_RS21655	4501057..4501959	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FNE87_RS21660	4501956..4502591	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FNE87_RS21665	4502588..4503517	+	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
FNE87_RS21670	4503847..4504089	-	243	WP_001086388.1	hypothetical protein	-
FNE87_RS21675	4504308..4504526	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FNE87_RS21680	4505131..4505421	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FNE87_RS21685	4505422..4505733	-	312	WP_000897305.1	hypothetical protein	Toxin
FNE87_RS21690	4505962..4506870	+	909	WP_001162704.1	alpha/beta hydrolase	-
FNE87_RS21695	4506934..4507875	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FNE87_RS21700	4507920..4508357	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FNE87_RS21705	4508354..4509226	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FNE87_RS21710	4509220..4509819	-	600	WP_001315111.1	glucose-1-phosphatase	-
FNE87_RS21715	4509918..4510703	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T128375 WP_000897305.1 NZ_CP041416:c4505733-4505422 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128375 NZ_CP041416:c4505733-4505422 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128375 WP_000356397.1 NZ_CP041416:c4505421-4505131 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128375 NZ_CP041416:c4505421-4505131 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7