Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2017938..2018440 Replicon chromosome
Accession NZ_CP041416
Organism Escherichia coli strain STEC711

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FNE87_RS09665 Protein ID WP_000767829.1
Coordinates 2018186..2018440 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNE87_RS09660 Protein ID WP_001259255.1
Coordinates 2017938..2018189 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE87_RS09635 2013116..2014183 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FNE87_RS09640 2014183..2015253 - 1071 WP_050864004.1 histidinol-phosphate transaminase -
FNE87_RS09645 2015250..2016554 - 1305 WP_050864003.1 histidinol dehydrogenase -
FNE87_RS09650 2016560..2017459 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FNE87_RS09655 2017605..2017655 - 51 WP_001364200.1 his operon leader peptide -
FNE87_RS09660 2017938..2018189 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNE87_RS09665 2018186..2018440 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNE87_RS09670 2018523..2019347 + 825 WP_000754749.1 SDR family oxidoreductase -
FNE87_RS09675 2019393..2020322 + 930 WP_000803351.1 LysR family transcriptional regulator -
FNE87_RS09680 2020537..2020599 + 63 WP_010723108.1 membrane protein YoeI -
FNE87_RS09685 2020589..2021947 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FNE87_RS09690 2022126..2023184 + 1059 WP_000492346.1 transport protein YeeE -
FNE87_RS09695 2023198..2023425 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128356 WP_000767829.1 NZ_CP041416:2018186-2018440 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128356 NZ_CP041416:2018186-2018440 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128356 WP_001259255.1 NZ_CP041416:2017938-2018189 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128356 NZ_CP041416:2017938-2018189 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References