Detailed information of TA system TA128343

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62360..62629
• Replicon: plasmid pSTEC719_4
• Accession: NZ_CP041415
• Organism: Escherichia coli strain STEC719

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FNE88_RS25635
• Protein ID: WP_001312861.1
• Coordinates: 62471..62629 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	62360..62425 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE88_RS25605	58127..58666	+	540	WP_000290791.1	single-stranded DNA-binding protein	-
FNE88_RS25610	58729..58962	+	234	WP_000005990.1	DUF905 family protein	-
FNE88_RS25615	59028..60986	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
FNE88_RS25620	61041..61475	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
FNE88_RS25625	61472..62191	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
FNE88_RS25630	62203..62391	-	189	WP_001299721.1	hypothetical protein	-
-	62203..62427	+	225	NuclAT_0	-	-
-	62203..62427	+	225	NuclAT_0	-	-
-	62203..62427	+	225	NuclAT_0	-	-
-	62203..62427	+	225	NuclAT_0	-	-
-	62360..62425	+	66	-	-	Antitoxin
FNE88_RS25635	62471..62629	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FNE88_RS25645	63551..63838	+	288	WP_161622521.1	hypothetical protein	-
FNE88_RS25650	63956..64777	+	822	WP_001234469.1	DUF945 domain-containing protein	-
FNE88_RS25655	65074..65664	-	591	WP_165899088.1	transglycosylase SLT domain-containing protein	-
FNE88_RS25660	66015..66398	+	384	WP_001063021.1	relaxosome protein TraM	-
FNE88_RS25665	66589..67236	+	648	WP_000332519.1	conjugal transfer transcriptional regulator TraJ	-
FNE88_RS25670	67372..67587	+	216	WP_000086381.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..85426	85426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T128343 WP_001312861.1 NZ_CP041415:62471-62629 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128343 NZ_CP041415:62471-62629 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT128343 NZ_CP041415:62360-62425 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin