Detailed information of TA system TA128336

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13540..13794
• Replicon: plasmid pSTEC719_4
• Accession: NZ_CP041415
• Organism: Escherichia coli strain STEC719

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FNE88_RS25320
• Protein ID: WP_001351576.1
• Coordinates: 13588..13794 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	13540..13601 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE88_RS25305	11325..12071	+	747	WP_136769175.1	conjugal transfer pilus acetylase TraX	-
FNE88_RS25310	12126..12686	+	561	WP_001587438.1	fertility inhibition protein FinO	-
FNE88_RS25315	12822..13019	+	198	WP_097464675.1	hypothetical protein	-
FNE88_RS25965	13210..13284	+	75	Protein_11	endonuclease	-
-	13540..13601	-	62	NuclAT_1	-	Antitoxin
-	13540..13601	-	62	NuclAT_1	-	Antitoxin
-	13540..13601	-	62	NuclAT_1	-	Antitoxin
-	13540..13601	-	62	NuclAT_1	-	Antitoxin
FNE88_RS25320	13588..13794	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
FNE88_RS25325	14078..14335	+	258	WP_000083845.1	replication regulatory protein RepA	-
FNE88_RS25330	14567..14641	+	75	WP_001442103.1	RepA leader peptide Tap	-
FNE88_RS25335	14634..15116	+	483	WP_001523473.1	hypothetical protein	-
FNE88_RS25340	15109..15966	+	858	WP_161622518.1	incFII family plasmid replication initiator RepA	-
FNE88_RS25345	16137..16814	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
FNE88_RS25350	16814..17161	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FNE88_RS25355	17181..18752	+	1572	WP_000381395.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..85426	85426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T128336 WP_001351576.1 NZ_CP041415:13588-13794 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T128336 NZ_CP041415:13588-13794 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT128336 NZ_CP041415:c13601-13540 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin