Detailed information of TA system TA128331

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79538..79899
• Replicon: plasmid pSTEC719_3
• Accession: NZ_CP041414
• Organism: Escherichia coli strain STEC719

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: FNE88_RS25225
• Protein ID: WP_000775238.1
• Coordinates: 79738..79899 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	79538..79672 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE88_RS25950	74732..74938	+	207	WP_061361210.1	hypothetical protein	-
FNE88_RS25195	74964..75500	+	537	WP_161622503.1	single-stranded DNA-binding protein	-
FNE88_RS25200	75558..75791	+	234	WP_161622504.1	DUF905 family protein	-
FNE88_RS25205	75861..77825	+	1965	WP_161622505.1	ParB/RepB/Spo0J family partition protein	-
FNE88_RS25210	77894..78328	+	435	WP_050940544.1	conjugation system SOS inhibitor PsiB	-
FNE88_RS25215	78325..79044	+	720	WP_161622506.1	plasmid SOS inhibition protein A	-
FNE88_RS25220	79044..79562	+	519	WP_161622507.1	hypothetical protein	-
-	79538..79672	+	135	NuclAT_0	-	Antitoxin
-	79538..79672	+	135	NuclAT_0	-	Antitoxin
-	79538..79672	+	135	NuclAT_0	-	Antitoxin
-	79538..79672	+	135	NuclAT_0	-	Antitoxin
FNE88_RS25225	79738..79899	+	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
FNE88_RS25955	80575..80778	+	204	Protein_97	single-stranded DNA-binding protein	-
FNE88_RS25235	80801..81124	+	324	WP_161622508.1	hypothetical protein	-
FNE88_RS25240	81183..81425	+	243	WP_161622509.1	hypothetical protein	-
FNE88_RS25960	82121..82327	+	207	WP_061361210.1	hypothetical protein	-
FNE88_RS25255	82351..82647	+	297	WP_001272251.1	hypothetical protein	-
FNE88_RS25260	82757..83578	+	822	WP_001234447.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD	1..84140	84140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T128331 WP_000775238.1 NZ_CP041414:79738-79899 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T128331 NZ_CP041414:79738-79899 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 135 bp

>AT128331 NZ_CP041414:79538-79672 [Escherichia coli]
CAGGGTGCAGATATATGGGCTATGATGTGTCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGT
GTTGTGTGGCAGAAGGCAGAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin