Detailed information of TA system TA128330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4783094..4783696
• Replicon: chromosome
• Accession: NZ_CP041411
• Organism: Escherichia coli strain STEC719

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FNE88_RS23300
• Protein ID: WP_000897305.1
• Coordinates: 4783385..4783696 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FNE88_RS23295
• Protein ID: WP_000356397.1
• Coordinates: 4783094..4783384 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE88_RS23270	4779020..4779922	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FNE88_RS23275	4779919..4780554	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FNE88_RS23280	4780551..4781480	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FNE88_RS23285	4781810..4782052	-	243	Protein_4557	hypothetical protein	-
FNE88_RS23290	4782271..4782489	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FNE88_RS23295	4783094..4783384	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FNE88_RS23300	4783385..4783696	-	312	WP_000897305.1	hypothetical protein	Toxin
FNE88_RS23305	4783925..4784833	+	909	WP_001162704.1	alpha/beta hydrolase	-
FNE88_RS23310	4784897..4785838	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FNE88_RS23315	4785883..4786320	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FNE88_RS23320	4786317..4787189	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FNE88_RS23325	4787183..4787782	-	600	WP_001315111.1	glucose-1-phosphatase	-
FNE88_RS23330	4787881..4788666	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T128330 WP_000897305.1 NZ_CP041411:c4783696-4783385 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128330 NZ_CP041411:c4783696-4783385 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128330 WP_000356397.1 NZ_CP041411:c4783384-4783094 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128330 NZ_CP041411:c4783384-4783094 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7