Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3961371..3961912 Replicon chromosome
Accession NZ_CP041411
Organism Escherichia coli strain STEC719

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FNE88_RS19585 Protein ID WP_000615976.1
Coordinates 3961634..3961912 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FNE88_RS19580 Protein ID WP_000729704.1
Coordinates 3961371..3961631 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE88_RS19555 3957154..3957939 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
FNE88_RS19560 3957911..3959623 + 1713 Protein_3837 flagellar biosynthesis protein FlhA -
FNE88_RS19565 3959728..3960006 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FNE88_RS19570 3959999..3960355 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FNE88_RS19575 3960412..3961185 - 774 WP_000543895.1 C40 family peptidase -
FNE88_RS19580 3961371..3961631 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FNE88_RS19585 3961634..3961912 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FNE88_RS19590 3962068..3962808 + 741 WP_001225679.1 murein L,D-transpeptidase -
FNE88_RS19595 3962779..3963546 - 768 WP_000333380.1 class II glutamine amidotransferase -
FNE88_RS19600 3963752..3964330 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3959999..3972698 12699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T128328 WP_000615976.1 NZ_CP041411:3961634-3961912 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128328 NZ_CP041411:3961634-3961912 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT128328 WP_000729704.1 NZ_CP041411:3961371-3961631 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128328 NZ_CP041411:3961371-3961631 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References