Detailed information of TA system TA128305

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19284..19548
• Replicon: plasmid p45407_2
• Accession: NZ_CP041394
• Organism: Escherichia coli strain ECOL-18-VL-LA-PA-Ryan-0026

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: E3Z24_RS24385
• Protein ID: WP_001303307.1
• Coordinates: 19396..19548 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	19284..19346 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z24_RS24370	15386..16456	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
E3Z24_RS24375	16475..17683	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	17863..17923	-	61	NuclAT_3	-	-
-	17863..17923	-	61	NuclAT_3	-	-
-	17863..17923	-	61	NuclAT_3	-	-
-	17863..17923	-	61	NuclAT_3	-	-
E3Z24_RS24380	17990..19081	-	1092	WP_000426061.1	hypothetical protein	-
-	19284..19346	-	63	NuclAT_1	-	Antitoxin
-	19284..19346	-	63	NuclAT_1	-	Antitoxin
-	19284..19346	-	63	NuclAT_1	-	Antitoxin
-	19284..19346	-	63	NuclAT_1	-	Antitoxin
E3Z24_RS24385	19396..19548	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
E3Z24_RS24390	19620..19871	-	252	WP_001291968.1	hypothetical protein	-
-	20258..20309	-	52	NuclAT_4	-	-
-	20258..20309	-	52	NuclAT_4	-	-
-	20258..20309	-	52	NuclAT_4	-	-
-	20258..20309	-	52	NuclAT_4	-	-
E3Z24_RS25415	20795..20971	-	177	WP_001054900.1	hypothetical protein	-
E3Z24_RS24405	21180..21389	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
E3Z24_RS24410	21487..22101	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
E3Z24_RS24415	22177..24345	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1D / floR / ant(3'')-Ia / aac(3)-IId	-	1..137828	137828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T128305 WP_001303307.1 NZ_CP041394:19396-19548 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T128305 NZ_CP041394:19396-19548 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT128305 NZ_CP041394:c19346-19284 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin