Detailed information of TA system TA128294

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3194..3615
• Replicon: plasmid p45407_1
• Accession: NZ_CP041393
• Organism: Escherichia coli strain ECOL-18-VL-LA-PA-Ryan-0026

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: E3Z24_RS23465
• Protein ID: WP_001302184.1
• Coordinates: 3457..3615 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	3194..3392 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z24_RS23435	530..634	-	105	Protein_1	thiosulfate reductase cytochrome B subunit	-
E3Z24_RS23440	691..1384	+	694	Protein_2	IS1 family transposase	-
E3Z24_RS23445	1400..2023	+	624	WP_000912556.1	potassium channel family protein	-
E3Z24_RS23450	2108..2359	+	252	Protein_4	iron-containing alcohol dehydrogenase	-
E3Z24_RS23455	2648..2965	-	318	Protein_5	IS110 family transposase	-
E3Z24_RS23460	3120..3225	+	106	Protein_6	plasmid SOS inhibition protein A	-
-	3194..3392	+	199	NuclAT_0	-	Antitoxin
-	3194..3392	+	199	NuclAT_0	-	Antitoxin
-	3194..3392	+	199	NuclAT_0	-	Antitoxin
-	3194..3392	+	199	NuclAT_0	-	Antitoxin
E3Z24_RS23465	3457..3615	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3Z24_RS25390	4283..4489	+	207	WP_000275854.1	hypothetical protein	-
E3Z24_RS23475	4514..4801	+	288	WP_000107242.1	hypothetical protein	-
E3Z24_RS23480	4920..5741	+	822	WP_001234465.1	DUF945 domain-containing protein	-
E3Z24_RS23485	6037..6609	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
E3Z24_RS23490	6979..7362	+	384	WP_001063020.1	relaxosome protein TraM	-
E3Z24_RS23495	7553..8200	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
E3Z24_RS23500	8336..8551	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aadA5 / qacE / sul1 / blaNDM-5 / dfrA12 / aadA2 / mph(A)	-	1..139547	139547
	-	flank	IS/Tn	-	-	1007..1384	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T128294 WP_001302184.1 NZ_CP041393:3457-3615 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128294 NZ_CP041393:3457-3615 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT128294 NZ_CP041393:3194-3392 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin