Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3802334..3802875 Replicon chromosome
Accession NZ_CP041392
Organism Escherichia coli strain ECOL-18-VL-LA-PA-Ryan-0026

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag E3Z24_RS18415 Protein ID WP_000615983.1
Coordinates 3802597..3802875 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag E3Z24_RS18410 Protein ID WP_000729703.1
Coordinates 3802334..3802594 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3Z24_RS18390 3798009..3798794 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
E3Z24_RS18395 3798766..3800478 + 1713 Protein_3563 flagellar biosynthesis protein FlhA -
E3Z24_RS18400 3800702..3801199 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
E3Z24_RS18405 3801375..3802124 - 750 WP_000056849.1 C40 family peptidase -
E3Z24_RS18410 3802334..3802594 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
E3Z24_RS18415 3802597..3802875 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
E3Z24_RS18420 3803031..3803771 + 741 WP_001225679.1 murein L,D-transpeptidase -
E3Z24_RS18425 3803742..3804509 - 768 WP_000333380.1 class II glutamine amidotransferase -
E3Z24_RS18430 3804715..3805293 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T128290 WP_000615983.1 NZ_CP041392:3802597-3802875 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128290 NZ_CP041392:3802597-3802875 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT128290 WP_000729703.1 NZ_CP041392:3802334-3802594 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128290 NZ_CP041392:3802334-3802594 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References