Detailed information of TA system TA128289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3796137..3796831
• Replicon: chromosome
• Accession: NZ_CP041392
• Organism: Escherichia coli strain ECOL-18-VL-LA-PA-Ryan-0026

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: E3Z24_RS18375
• Protein ID: WP_001263489.1
• Coordinates: 3796137..3796535 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: E3Z24_RS18380
• Protein ID: WP_000554758.1
• Coordinates: 3796538..3796831 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3791725..3791805	-	81	NuclAT_12	-	-
-	3791725..3791805	-	81	NuclAT_12	-	-
-	3791725..3791805	-	81	NuclAT_12	-	-
-	3791725..3791805	-	81	NuclAT_12	-	-
E3Z24_RS18350	3792401..3792859	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E3Z24_RS18355	3793120..3794577	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
E3Z24_RS18360	3794634..3795155	-	522	Protein_3556	peptide chain release factor H	-
E3Z24_RS18365	3795151..3795357	-	207	Protein_3557	RtcB family protein	-
E3Z24_RS18370	3795675..3796127	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
E3Z24_RS18375	3796137..3796535	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E3Z24_RS18380	3796538..3796831	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E3Z24_RS18385	3796883..3797938	-	1056	WP_001226164.1	DNA polymerase IV	-
E3Z24_RS18390	3798009..3798794	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
E3Z24_RS18395	3798766..3800478	+	1713	Protein_3563	flagellar biosynthesis protein FlhA	-
E3Z24_RS18400	3800702..3801199	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T128289 WP_001263489.1 NZ_CP041392:c3796535-3796137 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T128289 NZ_CP041392:c3796535-3796137 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT128289 WP_000554758.1 NZ_CP041392:c3796831-3796538 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT128289 NZ_CP041392:c3796831-3796538 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3