Detailed information of TA system TA128261

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 133876..134129
• Replicon: plasmid pKP58-2
• Accession: NZ_CP041375
• Organism: Klebsiella pneumoniae strain KP58

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FM021_RS29505
• Protein ID: WP_001312851.1
• Coordinates: 133980..134129 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	133876..133935 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FM021_RS29455	129258..129602	-	345	Protein_158	IS6-like element IS26 family transposase	-
FM021_RS29465	129654..130358	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FM021_RS29470	130422..130583	+	162	Protein_160	DNA helicase	-
FM021_RS29475	130603..131349	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
FM021_RS29480	131404..131964	+	561	WP_000139328.1	fertility inhibition protein FinO	-
FM021_RS29485	132096..132296	+	201	WP_015059022.1	hypothetical protein	-
FM021_RS29490	132682..133281	+	600	WP_032083981.1	hypothetical protein	-
FM021_RS29495	133445..133675	+	231	WP_001736714.1	hypothetical protein	-
-	133876..133935	-	60	NuclAT_1	-	Antitoxin
-	133876..133935	-	60	NuclAT_1	-	Antitoxin
-	133876..133935	-	60	NuclAT_1	-	Antitoxin
-	133876..133935	-	60	NuclAT_1	-	Antitoxin
FM021_RS29505	133980..134129	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
FM021_RS29510	134413..134661	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..134972	134972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T128261 WP_001312851.1 NZ_CP041375:133980-134129 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T128261 NZ_CP041375:133980-134129 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT128261 NZ_CP041375:c133935-133876 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin