Detailed information of TA system TA128254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 40317..41044
• Replicon: plasmid pKP58-1
• Accession: NZ_CP041374
• Organism: Klebsiella pneumoniae strain KP58

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: FM021_RS27575
• Protein ID: WP_011251285.1
• Coordinates: 40733..41044 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FM021_RS27570
• Protein ID: WP_011251286.1
• Coordinates: 40317..40736 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FM021_RS27535	35485..35955	-	471	WP_048333570.1	hypothetical protein	-
FM021_RS30745	36148..36903	-	756	Protein_37	hypothetical protein	-
FM021_RS27545	37499..37942	+	444	WP_048333569.1	hypothetical protein	-
FM021_RS27550	37963..38751	+	789	WP_040217257.1	hypothetical protein	-
FM021_RS27555	38765..39100	+	336	Protein_40	conjugal transfer protein TraI	-
FM021_RS27560	39202..40170	-	969	WP_074428168.1	IS5 family transposase	-
FM021_RS27570	40317..40736	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
FM021_RS27575	40733..41044	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
FM021_RS27580	41249..41707	-	459	Protein_44	DDE-type integrase/transposase/recombinase	-
FM021_RS27585	41821..42518	+	698	Protein_45	IS1 family transposase	-
FM021_RS27590	43904..44853	-	950	Protein_46	DUF1173 family protein	-
FM021_RS27595	45031..45525	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..197415	197415
	-	inside	IScluster/Tn	-	-	22688..47711	25023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T128254 WP_011251285.1 NZ_CP041374:c41044-40733 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T128254 NZ_CP041374:c41044-40733 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT128254 WP_011251286.1 NZ_CP041374:c40736-40317 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT128254 NZ_CP041374:c40736-40317 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1