Detailed information of TA system TA128224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4207694..4208510
• Replicon: chromosome
• Accession: NZ_CP041362
• Organism: Citrobacter amalonaticus strain 133355-SW-C4-Cam

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: FEO47_RS20080
• Protein ID: WP_142765510.1
• Coordinates: 4207694..4208170 (-)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A3Q8DNJ3
• Locus tag: FEO47_RS20085
• Protein ID: WP_042999108.1
• Coordinates: 4208175..4208510 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEO47_RS20060	4202799..4203542	-	744	WP_044264050.1	molecular chaperone	-
FEO47_RS20065	4203585..4206059	-	2475	WP_044257386.1	fimbria/pilus outer membrane usher protein	-
FEO47_RS20070	4206123..4206572	-	450	WP_086513030.1	type 1 fimbrial protein	-
FEO47_RS20075	4206740..4207270	-	531	WP_042999110.1	type 1 fimbrial protein	-
FEO47_RS20080	4207694..4208170	-	477	WP_142765510.1	type II toxin-antitoxin system YhaV family toxin	Toxin
FEO47_RS20085	4208175..4208510	-	336	WP_042999108.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
FEO47_RS20090	4208725..4209261	-	537	WP_142765433.1	ferredoxin-type protein NapF	-
FEO47_RS20095	4209258..4209854	-	597	WP_142765434.1	molecular chaperone	-
FEO47_RS20100	4209899..4210672	-	774	WP_044257303.1	dimethyl sulfoxide reductase anchor subunit	-
FEO47_RS20105	4210665..4211291	-	627	WP_042999104.1	dimethylsulfoxide reductase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (15-110)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 18223.81 Da
• Isoelectric Point: 8.8956

>T128224 WP_142765510.1 NZ_CP041362:c4208170-4207694 [Citrobacter amalonaticus]
MDFPTWLNGWTIYAHPCFIDQYNALVARVEDLKHRFPDPIVFQKKKESKVLAHLLKSIANITHEPRASVYRPGDSIGKAY
TDWSRAKFGGGRYRLFFRYSLESKIIVIAWVNDEGPLRTYGSKTDAYKIFGKMLDEGNPPDDWLSLLQACQNDGKEHL

Nucleotide

• Length: 477 bp

>T128224 NZ_CP041362:c4208170-4207694 [Citrobacter amalonaticus]
ATGGATTTTCCCACATGGCTGAACGGGTGGACGATCTACGCTCATCCCTGTTTTATCGATCAATACAACGCGCTGGTCGC
ACGTGTTGAAGACCTTAAGCACAGGTTTCCAGATCCCATTGTTTTCCAGAAGAAGAAAGAGAGCAAGGTGCTGGCGCATT
TATTGAAATCCATTGCCAACATTACCCACGAACCCCGAGCTTCTGTTTACCGGCCTGGTGACAGTATAGGGAAAGCTTAT
ACAGACTGGTCGAGAGCGAAGTTTGGTGGAGGACGGTATCGCTTATTTTTCCGTTATAGTCTCGAGAGTAAGATCATTGT
CATCGCATGGGTGAATGATGAAGGGCCATTGCGCACCTACGGCAGCAAGACCGATGCCTATAAGATTTTCGGGAAAATGC
TCGATGAGGGGAATCCACCCGACGACTGGCTAAGTTTGCTTCAGGCTTGTCAAAACGACGGGAAGGAACATCTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.83 Da
• Isoelectric Point: 4.1876

>AT128224 WP_042999108.1 NZ_CP041362:c4208510-4208175 [Citrobacter amalonaticus]
MFAYVRSEDIAIEADSKLTERHQTTIPASIRDALHLRSGDRIHYTLLSTGEVLLSKYSEATEDGVMRSFLDFLSQDVANN
PQTLKKLDLSRGYELVDGIDVNLDDEISDED

Nucleotide

• Length: 336 bp

>AT128224 NZ_CP041362:c4208510-4208175 [Citrobacter amalonaticus]
ATGTTCGCCTATGTTCGATCTGAAGACATTGCCATTGAAGCGGATTCTAAATTGACGGAACGACATCAAACGACTATCCC
TGCGTCGATCAGGGACGCTTTACATCTTCGCAGTGGTGATCGTATTCATTACACATTGCTTTCCACGGGTGAAGTACTGC
TTTCAAAATACAGTGAAGCAACGGAGGACGGTGTGATGCGATCATTCCTGGACTTCTTATCACAGGATGTGGCGAATAAC
CCACAAACGCTGAAGAAGCTGGATTTATCCCGTGGCTATGAACTGGTTGATGGTATCGACGTCAATCTTGATGATGAAAT
CAGCGACGAAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q8DNJ3