Detailed information of TA system TA128190

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3788215..3788586
• Replicon: chromosome
• Accession: NZ_CP041359
• Organism: Escherichia coli strain U1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: E3Z32_RS19525
• Protein ID: WP_001317028.1
• Coordinates: 3788392..3788586 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3788215..3788393 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z32_RS19500	3784170..3785543	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
E3Z32_RS19505	3785672..3786607	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
E3Z32_RS19510	3786659..3787894	-	1236	WP_001551035.1	site-specific integrase	-
E3Z32_RS19515	3787896..3788111	-	216	WP_000079604.1	excisionase XisR	-
E3Z32_RS19520	3788211..3788399	-	189	WP_001551036.1	DUF1187 family protein	-
-	3788215..3788393	+	179	NuclAT_0	-	Antitoxin
-	3788215..3788393	+	179	NuclAT_0	-	Antitoxin
-	3788215..3788393	+	179	NuclAT_0	-	Antitoxin
-	3788215..3788393	+	179	NuclAT_0	-	Antitoxin
E3Z32_RS19525	3788392..3788586	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E3Z32_RS19530	3788643..3789452	-	810	WP_001551037.1	recombination protein RecT	-
E3Z32_RS19535	3789445..3792096	-	2652	WP_001551038.1	exodeoxyribonuclease VIII	-
E3Z32_RS19540	3792198..3792473	-	276	WP_001314664.1	hypothetical protein	-
E3Z32_RS19545	3792548..3792718	-	171	WP_001551041.1	phage protein	-
E3Z32_RS19550	3792718..3792939	-	222	WP_000560221.1	killing protein KilR	-
E3Z32_RS19555	3793360..3793512	-	153	WP_001551042.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3786659..3833949	47290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T128190 WP_001317028.1 NZ_CP041359:c3788586-3788392 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T128190 NZ_CP041359:c3788586-3788392 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT128190 NZ_CP041359:3788215-3788393 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin