Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2586634..2587328 Replicon chromosome
Accession NZ_CP041359
Organism Escherichia coli strain U1

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag E3Z32_RS13675 Protein ID WP_001263491.1
Coordinates 2586930..2587328 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag E3Z32_RS13670 Protein ID WP_000554755.1
Coordinates 2586634..2586927 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3Z32_RS13645 2582255..2582611 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
E3Z32_RS13650 2582604..2582882 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
E3Z32_RS13655 2582987..2584699 - 1713 Protein_2417 flagellar biosynthesis protein FlhA -
E3Z32_RS13660 2584671..2585456 + 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
E3Z32_RS13665 2585527..2586582 + 1056 WP_001550655.1 DNA polymerase IV -
E3Z32_RS13670 2586634..2586927 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E3Z32_RS13675 2586930..2587328 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E3Z32_RS13680 2587338..2587790 + 453 WP_019842500.1 GNAT family N-acetyltransferase -
E3Z32_RS13685 2588036..2588242 + 207 Protein_2423 RtcB family protein -
E3Z32_RS27420 2588238..2588590 + 353 Protein_2424 peptide chain release factor H -
E3Z32_RS13695 2588647..2590104 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
E3Z32_RS13700 2590365..2590823 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
E3Z32_RS13705 2590915..2592159 + 1245 WP_001550657.1 esterase FrsA -
- 2591419..2591499 + 81 NuclAT_11 - -
- 2591419..2591499 + 81 NuclAT_11 - -
- 2591419..2591499 + 81 NuclAT_11 - -
- 2591419..2591499 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T128185 WP_001263491.1 NZ_CP041359:2586930-2587328 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T128185 NZ_CP041359:2586930-2587328 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT128185 WP_000554755.1 NZ_CP041359:2586634-2586927 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT128185 NZ_CP041359:2586634-2586927 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References