Detailed information of TA system TA128164

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47938..48207
• Replicon: plasmid unnamed1
• Accession: NZ_CP041358
• Organism: Escherichia coli strain U1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: E3Z32_RS00360
• Protein ID: WP_001312861.1
• Coordinates: 48049..48207 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47938..48003 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z32_RS00335	43707..44234	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
E3Z32_RS00340	44292..44525	+	234	WP_000006003.1	DUF905 family protein	-
E3Z32_RS00345	44586..46550	+	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
E3Z32_RS00350	46619..47053	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
E3Z32_RS00355	47050..47769	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	47781..48005	+	225	NuclAT_0	-	-
-	47781..48005	+	225	NuclAT_0	-	-
-	47781..48005	+	225	NuclAT_0	-	-
-	47781..48005	+	225	NuclAT_0	-	-
E3Z32_RS27135	47790..47969	-	180	WP_001309233.1	hypothetical protein	-
-	47938..48003	+	66	-	-	Antitoxin
E3Z32_RS00360	48049..48207	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3Z32_RS00365	48508..48712	-	205	Protein_59	pilus protein	-
E3Z32_RS00375	49104..49400	+	297	WP_001545326.1	hypothetical protein	-
E3Z32_RS00380	49510..50331	+	822	WP_001234445.1	DUF945 domain-containing protein	-
E3Z32_RS00385	50628..51230	-	603	Protein_62	transglycosylase SLT domain-containing protein	-
E3Z32_RS00390	51551..51934	+	384	WP_001151538.1	relaxosome protein TraM	-
E3Z32_RS00395	52121..52810	+	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..130003	130003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T128164 WP_001312861.1 NZ_CP041358:48049-48207 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128164 NZ_CP041358:48049-48207 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT128164 NZ_CP041358:47938-48003 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin