Detailed information of TA system TA128161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5466386..5466981
• Replicon: chromosome
• Accession: NZ_CP041354
• Organism: Pseudomonas aeruginosa strain AZPAE15042

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: IHMA87_RS26300
• Protein ID: WP_003113526.1
• Coordinates: 5466703..5466981 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IHMA87_RS26295
• Protein ID: WP_034079919.1
• Coordinates: 5466386..5466691 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHMA87_RS26270	5461390..5462490	+	1101	WP_003151685.1	redox-regulated ATPase YchF	-
IHMA87_RS26275	5462562..5463611	-	1050	WP_079383482.1	lipase secretion chaperone	-
IHMA87_RS26280	5463692..5464621	-	930	WP_012077421.1	triacylglycerol lipase	-
IHMA87_RS26285	5464889..5465998	+	1110	WP_170870656.1	AraC family transcriptional regulator	-
IHMA87_RS26295	5466386..5466691	-	306	WP_034079919.1	HigA family addiction module antidote protein	Antitoxin
IHMA87_RS26300	5466703..5466981	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IHMA87_RS26305	5467034..5467353	-	320	Protein_5066	tyrosine-type recombinase/integrase	-
IHMA87_RS26310	5467654..5467857	+	204	WP_033976173.1	hypothetical protein	-
IHMA87_RS26315	5468098..5469957	+	1860	WP_034079923.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T128161 WP_003113526.1 NZ_CP041354:c5466981-5466703 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T128161 NZ_CP041354:c5466981-5466703 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGATGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCCATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCTGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACAGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11170.58 Da
• Isoelectric Point: 4.4462

>AT128161 WP_034079919.1 NZ_CP041354:c5466691-5466386 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLTELDISPAALARALNVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT128161 NZ_CP041354:c5466691-5466386 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGACGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAACGTCTCCGCCCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGTTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3